Composite

Part:BBa_K1911004:Design

Designed by: Jack Kwon   Group: iGEM16_LambertGA   (2016-10-12)


pLamdaR-LacI-GFP-LAA-pLac-ClpXP-CI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1257
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1221
    Illegal BglII site found at 3115
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2597
    Illegal AgeI site found at 3335
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1900


Design Notes

We had to make sure there weren't any illegal restriction enzyme sites. Double terminators and double stop codons were added appropriately so that translation and transcription didn't continue to express random DNA sequences.

Source

The part was created by combining multiple parts from the iGEM registry.

References